(a) Histological findings of Toluidine blue staining (TB) and Hematoxylin and eosin (H–E) staining (HE) in each group with PLGA scaffolds at 1, 3 and 6 months after the transplantation. HE stain shows that there is no osseous structures formation in each group. Proteoglycan was observed as metachromasia in the constructs with PLGA/cells/iliac and constructs with PLGA/cells in TB stain. (b) The observation reveals that the areas of metachromasia increase with time.